Bio Efficacy of Biogenic Silver Nanoparticles against Rice Sheath Blight Causing Pathogen Rhizoctonia solani Kuhn

Abstract

Silver nano particles were synthesized using two potential isolates of Trichoderma, Psudomonas fluorescens and one isolate of pathogen Rhizoctonia solani cell free culture filtrates incubated at different days 0, 5, 10 and 15. The present investigation revealed that bionano preparations irrespective of the isolate source used, i.e., Trichoderma, P. fluorescens or R. solani were found better in decreasing the sheath blight incidence in vitro though variation existed in terms of quantum of disease reduction. Bionano preparation from nutrient broth alone was also found effective in decreasing sheath blight severity compared to bioagents used alone or PDB. When the sensitivity of rice leaves from cv. NLR 34449 was assessed by dipping the leaf segments in 100% concentration of bionano silver (prepared using 170ppm silver nitrate), rice leaves turned yellow from the third day of incubation in detached leaf method in vitro.