Robust, Highly Visible, and Facile Bioconjugation Colloidal Crystal Beads for Bioassay

Abstract

High mechanical strength, highly visible, and admirable grafting molecular ability is the key challenge for colloidal photonic crystal (CPC) barcode beads in multiplex analysis fields. To achieve this goal, we proposed self-adhesion particles, polydopamine-coated SiO₂ nanoparticles (PDA@SiO₂), to construct CPC barcode beads by droplet-based microfluidic approach. Because of the adhesion, broad absorption of light, and "active" functional groups of PDA, the beads are endowed with high robustness, visibility, and excellent biomolecule immobilization. Ultrasonic treatment and compression experiments demonstrated that PDA@SiO₂ CPC barcode beads have a high mechanical strength. Color analysis illustrated that PDA@SiO₂ CPC beads exhibited a high visibility in color. The verification of fluorescent-tagged biomolecule conjugation together with the antigen detection stated that PDA@SiO₂ CPC beads are capable of immobilizing biomolecule by covalent binding. With a sandwich format, the beads were applied to analyze the tumor makers including alpha fetal protein, carcinoembryonic antigen, and prostate specific antigen from practical clinical serum. The proposed suspension arrays using PDA@SiO₂ CPC beads as a barcode showed acceptable accuracy and detection reproducibility.