A Simple and Effective Colorimetric Assay for Glucose Based on MnO2 Nanosheets

Abstract

Simple and effective methods for the detection of the level of blood glucose are closely linked to the monitoring of people's health. In the study, MnO₂ nanosheets with absorption range of 300 nm~500 nm and obvious yellow color were easily prepared and applied to detect glucose through their absorbance and color. The proposed method is based on the fact that a specific concentration of glucose can be quantitatively transformed into hydrogen peroxide (H₂O₂) under the catalytic effect of glucose oxidase. Based on the redox reaction of MnO₂ with H₂O₂, yellow MnO₂ can be converted into colorless Mn²⁺ to monitor the concentration of glucose. Under optimal conditions, a simple and effective visual assay for the sensitive and reliable detection of glucose was developed. The linear range was estimated to the range from 0 μM to 100 μM, with a detection limit of 12.8 μM. Furthermore, the proposed colorimetric assay based on MnO₂ nanosheets can effectively detect blood glucose of clinical serum samples with accuracy and convenience.