The Immunometabolomic Interface Receptor Hydroxycarboxylic Acid Receptor 2 Mediates the Therapeutic Effects of Dimethyl Fumarate in Autoantibody-Induced Skin Inflammation

Abstract

The drug dimethyl fumarate (DMF) is in clinical use for the treatment of psoriasis and multiple sclerosis. In addition, it has recently been demonstrated to ameliorate skin pathology in mouse models of pemphigoid diseases, a group of autoimmune blistering diseases of the skin and mucous membranes. However, the mode of action of DMF in inflammatory skin diseases has remained elusive. Therefore, we have investigated here the mechanisms by which DMF improves skin pathology, using the antibody transfer model of bullous pemphigoid-like epidermolysis bullosa acquisita (EBA). Experimental EBA was induced by transfer of antibodies against collagen VII that triggered the infiltration of immune cells into the skin and led to inflammatory skin lesions. DMF treatment reduced the infiltration of neutrophils and monocytes into the skin explaining the improved disease outcome in DMF-treated animals. Upon ingestion, DMF is converted to monomethyl fumarate that activates the hydroxycarboxylic acid receptor 2 (HCA₂). Interestingly, neutrophils and monocytes expressed <i>Hca2</i>. To investigate whether the therapeutic effect of DMF in EBA is mediated by HCA₂, we administered oral DMF to <i>Hca2</i>-deficient mice (<i>Hca2</i>^-/-) and wild-type littermates (<i>Hca2</i>^+/+) and induced EBA. DMF treatment ameliorated skin lesions in <i>Hca2</i>^+/+ but not in <i>Hca2</i>^-/- animals. These findings demonstrate that HCA₂ is a molecular target of DMF treatment in EBA and suggest that HCA₂ activation limits skin pathology by inhibiting the infiltration of neutrophils and monocytes into the skin.