Gene expression in two contrasting hybrid clones of Eucalyptus camaldulensis x Eucalyptus urophylla grown under water deficit conditions

A new mathematical model for relative quantification in real-time RT-PCR

Michael W. Pfaffl · Nucleic Acids Research · 2001 · 34.9K citations · Full text

Life Sciences, Molecular Biology, Gene Expression +12

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

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Life Sciences, Molecular Biology, Molecular Genetics +14

Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets

Claus L. Andersen, Jens Ledet Jensen, Torben F. Ørntoft · Cancer Research · 2004 · 7.2K citations

Life Sciences, Biostatistics, Molecular Biology +17

Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

Michael W. Pfaffl, Aleš Tichopád, Christian Prgomet et al. · Biotechnology Letters · 2004 · 5.1K citations

Life Sciences, Genetics, Molecular Biology +12

A simple and efficient method for isolating RNA from pine trees

Shujun Chang, Jeff Puryear, John Cairney · Plant Molecular Biology Reporter · 1993 · 3.6K citations

Life Sciences, Molecular Biology, Plant Sciences +6