The transactivation domain of Pho4 is required for nucleosome disruption at the PHO5 promoter.

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

R Sikorski, P. Hieter · Genetics · 1989 · 8.8K citations · Full text

The "megaprimer" method of site-directed mutagenesis.

Gobinda Sarkar, S S Sommer · PubMed · 1990 · 1.2K citations

Engineering, Genetics, Oligonucleotide Primers +18

Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions

Alan M. Myers, Alexander Tzagoloff, D M Kinney et al. · Gene · 1986 · 618 citations

Engineering, Yeast Shuttle, Biotechnology +8

Removal of positioned nucleosomes from the yeast PHO5 promoter upon PHO5 induction releases additional upstream activating DNA elements.

A. Almer, Hans K. Rudolph, A. Hinnen et al. · The EMBO Journal · 1986 · 463 citations · Full text

Chromatin, Transcriptional Regulation, Chromatin Structure +15

Critical Structural Elements of the VP16 Transcriptional Activation Domain

W. Douglas Cress, Steven J. Triezenberg · Science · 1991 · 449 citations

Critical Structural Elements, Transcriptional Regulation, Activation Domain +15