The properties of antigenic preparations from <i>Brucella melitensis</i>

Abstract

IN the preceding papers [Miles & Pirie, 1939, 3, 4] stages in the hydrolysis of the antigen of Brucella melitensis were described, the final product being a com- plex amine. This amine is prepared from its formyl derivative by hydrolysis. Considerable discoloration occurs when hydrochloric or sulphuric acids are used for the hydrolysis; if nitric acid is used there is no discoloration but a partly deaminated product results. The free amine can be obtained nearly colourless