In vitro plant regeneration from male flowers of banana.

Abstract

Abstract The present study was undertaken with a view to establish a protocol on in vitro regeneration of plants by using young male flowers of banana ( Musa sp. cv. Sabri) as explant. Appropriate developmental stage of immature male flowers for inoculation, medium composition for induction of calli, regeneration of plants, rooting of in vitro regenerated shoots, acclimatization of in vitro regenerated plantlets and ex vitro establishments of plantlets were worked out. Young male flowers obtained by striping away the bracts in between 24 to 26 were found suitable as explants for induction of callus. The isolated male flowers were cultured on MS (Murashige and Skoog) medium supplemented with different concentrations and combinations of three auxins viz. 2, 4-D (2,4-Dichlorophenoxyacetic acid), NAA (Napthaleneacetic acid) and IAA (Indole-3-acetic acid) for induction of callus. Only two of the total twenty International Journal of Biosciences (IJB ) ISSN: 2220-6655 (Print) Vol. 1, No. 1, p. 1-11, 2011 http://www.innspub.net