Circulating ectosomes: Determination of angiogenic microRNAs in type 2 diabetes

Abstract

Ectosomes (<i>Ects</i>) are a subpopulation of extracellular vesicles formed by the process of plasma membrane shedding. In the present study, we profiled ectosome-specific microRNAs (miRNAs) in patients with type 2 diabetes mellitus (T2DM) and analyzed their pro- and anti-angiogenic potential. <b>Methods:</b> We used different approaches for detecting and enumerating <i>Ects</i>, including atomic force microscopy, cryogenic transmission electron microscopy, and nanoparticle tracking analysis. Furthermore, we used bioinformatics tools to analyze functional data obtained from specific miRNA enrichment signatures during angiogenesis and vasculature development. <b>Results:</b> Levels of miR-193b-3p, miR-199a-3p, miR-20a-3p, miR-26b-5p, miR-30b-5p, miR-30c-5p, miR-374a-5p, miR-409-3p, and miR-95-3p were significantly different between <i>Ects</i> obtained from patients with T2DM and those obtained from healthy controls. <b>Conclusion</b>: Our results showed differences in the abundance of pro- and anti-angiogenic miRNAs in <i>Ects</i> of patients with T2DM, and are suggestive of mechanisms underlying the development of vascular complications due to impaired angiogenesis in such patients.