Comparison of Tris egg yolk-based, Triladyl^®and Optixell^®extender on post-thaw quality, Kinematics and in vivo fertility of Nili Ravi Buffalo<i>(Bubalus bubalis)</i>bull spermatozoa

Abstract

Our objectives were to ascertain the comparison of Tris egg yolk-based, Triladyl® and Optixell® extender on postthaw quality, CASA parameters and in vivo fertility of Nili Ravi buffalo (Bubalus bubalis) bull spermatozoa. Semen samples (n = 35) from five bulls were diluted in Tris egg yolk-based, Triladyl®, Optixell® extender and frozen in 0.50 ml French straws. Postthaw sperm CASA motility (%) was higher (p < 0.05) in Optixell extender as compared to Triladyl and Tris egg yolk-based extender. Although sperm progressive motility (%), morphology (%), average path velocity (μm/s), straight line velocity (μm/s), curvilinear velocity (μm/s), amplitude of lateral head displacement (μm), beat cross-frequency (Hz), straightness (%), length of curvilinear path (μm), length of average path (μm), intact plasma and acrosome membrane (%), and DNA integrity (%) were higher (p < 0.05) in spermatozoa cryopreserved in Optixell® extender as compared to Tris egg yolk-based and Triladyl® extender. The fertility rates (68.18%, 45.45%, 55.4%) were higher (p < 0.05) in buffaloes inseminated with semen doses frozen in Optixell extender than the Tris egg yolk-based and Triladyl® extender respectively. It is concluded that Optixell® extender improves postthaw semen quality and fertility in Nili Ravi buffaloes.