Genetic and Phylogenetic Characteristics of Pasteurella multocida Isolates From Different Host Species

Abstract

<i>Pasteurella multocida</i> is a leading cause of respiratory diseases in many host species. To understand the genetic characteristics of <i>P. multocida</i> strains isolated from different host species, we sequenced the genomic DNA of <i>P. multocida</i> isolated from pigs and analyzed the genetic characteristics of strains from avian species, bovine species, pigs, and rabbits using whole genome sequence (WGS) data. Our results found that a capsular: lipopolysaccharide (LPS): multilocus sequence typing (MLST) genotype A: L1: ST129 (43.75%) was predominant in avian <i>P. multocida</i>; while genotypes B: L2: ST122 (60.00%) and A: L3: ST79 (30.00%) were predominate in bovine <i>P. multocida</i>; genotype D: L6: ST50 (37.50%) in porcine <i>P. multocida</i>; and genotype A: L3: ST9 (76.47%) in rabbit <i>P. multocida</i>. Comparative genomic analysis of <i>P. multocida</i> from different host species found that there are no genes in the <i>P. multocida</i> genome that are specific to any type of host. Phylogenetic analysis using either whole-genome single nucleotide polymorphisms (SNPs) or the set of SNPs present in all single-copy core genes across genomes showed that <i>P. multocida</i> strains with the same LPS genotype and MLST genotype were clustered together, suggesting the combining both the LPS and MLST typing schemes better explained the topology seen in the <i>P. multocida</i> phylogeny.