Overexpression of BraLTP2, a Lipid Transfer Protein of Brassica napus, Results in Increased Trichome Density and Altered Concentration of Secondary Metabolites

Abstract

Plant non-specific lipid transfer proteins (nsLTPs) belong to a large multigene family that possesses complex physiological functions. Trichomes are present on the aerial surfaces of most plants and include both glandular secretory hairs and non-glandular hairs. In this study, <i>BraLTP2</i> was isolated from <i>Brassica rapa</i> (<i>B. rapa</i>) and its function was characterized in the important oilseed crop <i>Brassica napus</i> (<i>B. napus</i>). <i>B. rapa</i> lipid transfer protein 2 (<i>BraLTP2</i>) belongs to the little-known Y class of nsLTPs and encodes a predicted secretory protein. In Pro_BraLTP2::GUS (β-glucuronidase) transgenic plants, strong GUS activity was observed in young leaves and roots, while low activity was observed in the anther. It is noteworthy that strong GUS activity was observed in trichomes of the first four leaves of 4-week-old and 8-week-old seedings, however, it disappeared in 12-week-old seedings. In transgenic plants expressing a BraLTP2::GFP (green fluorescent protein) fusion protein, GFP fluorescence localized in the extracellular space of epidermal cells and trichomes. Overexpression of <i>BraLTP2</i> in <i>B. napus</i> caused an increase in trichome number and altered the accumulation of secondary metabolites in leaves, including 43 upregulated secondary metabolites. Moreover, transgenic plants showed significantly increased activities of antioxidant enzymes. These results suggest that <i>BraLTP2</i>, a new <i>nsLTP</i> gene, may play a role in trichome development and the accumulation of secondary metabolites.