Concepedia

Bright quantum dots emitting at ∼1,600 nm in the NIR-IIb window for deep tissue fluorescence imaging

Mingxi Zhang, Jingying Yue, Ran Cui, Zhuoran Ma, Hao Wan, Feifei Wang, Shoujun Zhu, Ying Zhou, Yun Kuang, Yeteng Zhong,

Proceedings of the National Academy of Sciences · 2018 · 429 citations · 51 references

TL;DR

In vivo fluorescence imaging in the near‑IR IIb window (1,500–1,700 nm) offers high spatial and temporal resolution and deep tissue penetration for fundamental research and potential clinical translation. The study developed a bright ∼1,600‑nm emitting PbS/CdS quantum‑dot probe. The probe uses a CdS shell to chemically passivate the PbS core, preserving fluorescence after aqueous phase transfer for biocompatibility. The probe enabled millimeter‑deep, high‑speed (up to 60 fps) imaging with micrometer resolution in 2D and 3D modes, and it proved nontoxic and largely excreted within a month, making it suitable for preclinical animal studies.

Abstract

Significance In vivo fluorescence imaging in near IR-IIb window (1,500–1,700 nm) can provide high spatial and temporal resolution and deep tissue penetration for fundamental research and potential translations. Herein, a bright fluorescent probe emitting at ∼1,600 nm based on lead sulfide (PbS)/CdS quantum dots was developed. The CdS shell helped to chemically passivate and retain the high fluorescence of the PbS core after phase transfer to aqueous solutions for biocompatibility. The 1,600-nm emitting probe allowed noninvasive, millimeter-deep fluorescence imaging at high speeds up to 60 frames per second with micrometer-scale spatial resolution in 2D wide-field and 3D confocal modes. The probes were nontoxic and largely excreted over 1 month, providing a tool for in vivo research of preclinical animal models.