Enhanced Optics for Time-Resolved Singlet Oxygen Luminescence Detection

Abstract

Singlet oxygen luminescence dosimetry (SOLD) is a highly promising direct monitoring method for photodynamic therapy (PDT) in the treatment of cancer. Early SOLD systems have been hampered by inefficient excitation, poor optical collection and immature infrared single photon detection technology. We report carefully engineered improvements addressing all of these deficiencies. We use a supercontinuum source with a tunable filter to precisely target the peak absorption wavelength of the chosen photosensitizer; we have designed a compact and versatile optical package for precise alignment; we have successfully employed state-of-the-art superconducting photon counting technologies. Through these improvements, we can achieve histogram acquisition from a photosensitizer in solution test sample. This setup opens the pathway to physiological SOLD studies for PDT dosimetry.