Genetic and Biochemical Characterization of OXA-519, a Novel OXA-48-Like β-Lactamase

Abstract

A multidrug-resistant <i>Klebsiella pneumoniae</i> 1210 isolate with reduced carbapenem susceptibility revealed the presence of a novel plasmid-encoded <i>bla</i>_OXA-48-like gene, named <i>bla</i>_OXA-519 The 60.7-kb plasmid (pOXA-519) was similar to the IncL-OXA-48 prototypical plasmid except for a ca. 2-kb deletion due to an IS<i>1R</i> insertion. OXA-519 differed from OXA-48 by a Val120Leu substitution, which resulted in an overall reduced β-lactam-hydrolysis profile, except those for ertapenem and meropenem, which were increased. Thus, detection of OXA-519 producers using biochemical tests that monitor imipenem hydrolysis will be difficult.