Long non‑coding RNA LINC00857 promotes gastric cancer cell proliferation and predicts poor patient survival

Abstract

Gastric cancer (GC) is a common malignancy worldwide and its pathogenesis remains unclear. Long non-coding RNAs (lncRNAs) serve an important function in cancer development, therefore identification of functional lncRNAs in GC is required. The results of the present study demonstrate that an lncRNA, <i>LINC00857</i>, was increased in GC tissues compared with adjacent non-tumor tissues. Overexpression of <i>LINC00857</i> was positively associated with poor survival rate, as well as with the tumor size of patients with GC. <i>LINC00857</i> knockdown induced by specific small interfering RNAs significantly inhibited GC cell proliferation <i>in vitro</i>. Genome-wide analysis revealed that <i>LINC00857</i> knockdown deregulated the cell cycle. Western blot analysis confirmed that <i>LINC00857</i> knockdown decreased protein expression of cyclin D1 and cyclin E1 in GC cells. Taken together, the results indicated that <i>LINC00857</i> knockdown suppressed GC cell proliferation through deregulating the cell cycle, resulting in the downregulation of cyclin D1 and cyclin E1. Therefore, <i>LINC00857</i> expression may be an independent biomarker for the diagnosis and prognosis of GC.