Current Protocols in Microbiology · 2015 · 64 citations · 5 references
Life SciencesBiotechnologyCell BiologyMolecular VirologyVaccinologyVirologyAnimal Cell CultureCell CultureCell Culture TechnologyMammalian Cell CultureVaccine FormulationViral VaccinesCell Culture ConditionsCell Culture-based Vaccine ProductionCell-based VaccineVaccine ProductionViral CultivationViral CultureVirus Production
The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Protocols for the preparation, titration, and trypsinization of vaccinia virus stocks, as well as viral DNA preparation and virus purification methods are also included.
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