Surface-enhanced Raman spectroscopic analysis of N⁶-benzylaminopurine residue quantity in sprouts with gold nanoparticles

Abstract

A rapid and quantitative method for the determination of N⁶-Benzylademine (N⁶-BA) was established through the application of surface-enhanced Raman spectroscopy (SERS). The Raman peak intensities of N⁶-BA at 1002 cm^-1 positively correlated to N⁶-BA concentrations in sprout extracts. The R² reached 0.99, and RSDs calculated below 10% at the concentration range of 0.1 ∼5μg mL^-1. The average recoveries were 80.0% ∼ 98.2% for blank samples intentionally contaminated at differing levels of 0.04, 0.4, and 1 μg g^-1. The whole procedure, including sample preparation and SERS detection, did not exceed 30 min for a set of 6 samples. This study indicates that SERS is a promising technique for rapid tracing analysis and on-site testing of N⁶-BA.