Hydrogen Sulfide Alleviates Lipopolysaccharide‐Induced Diaphragm Dysfunction in Rats by Reducing Apoptosis and Inflammation through ROS/MAPK and TLR4/NF‐<i>κ</i>B Signaling Pathways

Abstract

Diaphragm dysfunction is an important clinical problem worldwide. Hydrogen sulfide (H₂S) is involved in many physiological and pathological processes in mammals. However, the effect and mechanism of H₂S in diaphragm dysfunction have not been fully elucidated. In this study, we detected that the level of H₂S was decreased in lipopolysaccharide- (LPS-) treated L6 cells. Treatment with H₂S increased the proliferation and viability of LPS-treated L6 cells. We found that H₂S decreased reactive oxygen species- (ROS-) induced apoptosis through the mitogen-activated protein kinase (MAPK) signaling pathway in LPS-treated L6 cells. Administration of H₂S alleviated LPS-induced inflammation by mediating the toll-like receptor-4 (TLR-4)/nuclear factor-kappa B (NF-<i>κ</i>B) signaling pathway in L6 cells. Furthermore, H₂S improved diaphragmatic function and structure through the reduction of inflammation and apoptosis in the diaphragm of septic rats. In conclusion, these findings indicate that H₂S ameliorates LPS-induced diaphragm dysfunction in rats by reducing apoptosis and inflammation through ROS/MAPK and TLR4/NF-<i>κ</i>B signaling pathways. Novel slow-releasing H₂S donors can be designed and applied for the treatment of diaphragm dysfunction.