MethodsX · 2018 · 43 citations · 3 references
A multiplex PCR was described to simultaneously detect <i>mcr-1</i> and frequently occurring carbapenem-resistant genes including <i>bla</i><sub>KPC</sub><i>, bla</i><sub>NDM</sub><i>, bla</i><sub>IMP</sub>, and <i>bla</i><sub>OXA-48-like</sub> in a single reaction. The PCR product sizes of these 4 carbapenem-resistant genes were 232 bp, 438 bp, 621 bp, and 798 bp for <i>bla</i><sub>IMP</sub>, <i>bla</i><sub>OXA-48-like</sub>, <i>bla</i><sub>NDM</sub>, and <i>bla</i><sub>KPC</sub>, respectively, whereas <i>mcr-1</i> revealed 1126 bp of PCR product. This protocol accurately detected those resistant genes in agreement with the reference strains, 127 local carbapenem-resistant Enterobacteriaceae, 8 <i>mcr-1</i> carrying Enterobacteriaceae, and 62 carbapenem-susceptible Enterobacteriaceae. This method will be useful for laboratory application and surveillance of carbapenem and/or colistin-resistant bacteria.
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Wantana Paveenkittiporn, Anusak Kerdsin, Sukanya Chokngam et al. · Diagnostic Microbiology and Infectious Disease · 2016 · 30 citations
Antimicrobial Resistance Gene, Health Sciences, Antibiotics +9