Nitrosospira sp. Govern Nitrous Oxide Emissions in a Tropical Soil Amended With Residues of Bioenergy Crop

Abstract

Organic vinasse, a residue produced during bioethanol production, increases nitrous oxide (N₂O) emissions when applied with inorganic nitrogen (N) fertilizer in soil. The present study investigated the role of the ammonia-oxidizing bacteria (AOB) community on the N₂O emissions in soils amended with organic vinasse (CV: concentrated and V: non-concentrated) plus inorganic N fertilizer. Soil samples and N₂O emissions were evaluated at 11, 19, and 45 days after fertilizer application, and the bacterial and archaea gene (<i>amo</i>A) encoding the ammonia monooxygenase enzyme, bacterial denitrifier (<i>nir</i>K, <i>nir</i>S, and <i>nos</i>Z) genes and total bacteria were quantified by real time PCR. We also employed a deep <i>amo</i>A amplicon sequencing approach to evaluate the effect of treatment on the community structure and diversity of the soil AOB community. Both vinasse types applied with inorganic N application increased the total N₂O emissions and the abundance of AOB. <i>Nitrosospira</i> sp. was the dominant AOB in the soil and was correlated with N₂O emissions. However, the diversity and the community structure of AOB did not change with vinasse and inorganic N fertilizer amendment. The results highlight the importance of residues and fertilizer management in sustainable agriculture and can be used as a reference and an input tool to determine good management practices for organic fertilization.