Isolongifolene Attenuates Oxidative Stress and Behavioral Impairment in Rotenone-Induced Rat Model of Parkinson’s Disease

Abstract

Introduction: Parkinson’s disease (PD) is a progressive age-related disease, in which dopaminergic neurons in the nigrostriatal pathway are destroyed, resulting in movement and behavioral impairment. Oxidative stress and the generation of reactive oxygen species play a key role in the progression and pathology of neurodegenerative diseases such as PD. Rotenone is a common pesticide that induces PD through the generation of oxidative stress. Isolongifolene (ILF), a tricyclic sesqueterpene of Murraya koenigii, has antioxidant and neuroprotective effects. The current study was aimed to investigate the effect of ILF against oxidative stress and movement impairment on rotenone-induced rat model of PD. Materials and Methods: Biochemical measures, including the activities of catalase, glutathione peroxidase (GPx), superoxide dismutase (SOD) and the levels of reduced glutathione (GSH) and lipid peroxidation products [thiobarbituric acid reactive substances (TBARS) and behavioral analysis (hang and catalepsy test) were performed. Results: The muscle strength and cataleptic score of the ILF co-treated groups were significantly improved. Treatment with ILF prevented the increases in the levels of TBARS, significantly improved the SOD, catalase, GPx activities, and GSH levels. Conclusion: These findings suggested that ILF has neuroprotective properties through its potent antioxidant activities.