The International Journal of Occupational and Environmental Medicine · 2018 · 21 citations · 23 references
PharmacyEnvironmental SciencesToxicologyEnvironmental ToxicologyIndustrial ToxicologyOccupational ToxicologyCellular ToxicologyChemical SafetyEnvironmental GenotoxicantsGenotoxicity TestingOccupational CarcinogenesisOccupational Genotoxic ExposureBuccal Cell Micronucleus AssayBuccal Micronucleus AssayComparison Group DesignCytome AssayMutagenic MechanismsOccupational Fume ExposureOccupational Inhalation ToxicityOccupational Inhalation Toxicology
BACKGROUND: Petrol pump workers are occupationally exposed to gasoline and its fumes consisting of several mutagenic chemicals. OBJECTIVE: To evaluate the genotoxic effects of exposure to gasoline fumes on petrol pump workers. METHODS: The study groups included 70 petrol pump workers (exposed group) and 70 healthy age-matched individuals with no known exposure (comparison group). Buccal micronucleus cytome assay (BMCyt) was performed to check the genotoxicity caused due to inhalation of gasoline fumes. RESULTS: The frequencies of micronucleated cells, nuclear bud, condensed chromatin cells, karyorrhectic cells, pyknotic cells, and karyolytic cells were significantly higher in the exposed workers compared to the comparison group. CONCLUSION: Exposure to gasoline fumes is associated with increased frequency of cell abnormalities. This may lead to various health consequences including cancer in those occupationally exposed to gasoline fumes.
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Cytokinesis-block micronucleus cytome assay
Michael Fenech · Nature Protocols · 2007 · 2K citations · Full text
Buccal micronucleus cytome assay
Philip Thomas, Nina Holland, Claudia Bolognesi et al. · Nature Protocols · 2009 · 631 citations