Contribution of central and peripheral adaptations to changes in maximal oxygen uptake following 4 weeks of sprint interval training

Abstract

The current study examined the contribution of central and peripheral adaptations to changes in maximal oxygen uptake (V̇O_2max) following sprint interval training (SIT). Twenty-three males completed 4 weekly SIT sessions (8 × 20-s cycling bouts at ∼170% of work rate at V̇O_2max, 10-s recovery) for 4 weeks. Following completion of training, the relationship between changes in V̇O_2max and changes in central (cardiac output) and peripheral (arterial-mixed venous oxygen difference (a-vO₂diff), muscle capillary density, oxidative capacity, fibre-type distribution) adaptations was determined in all participants using correlation analysis. Participants were then divided into tertiles on the basis of the magnitude of their individual V̇O_2max responses, and differences in central and peripheral adaptations were examined in the top (HI; ∼10 mL·kg^-1·min^-1 increase in V̇O_2max, p < 0.05) and bottom (LO; no change in V̇O_2max, p > 0.05) tertiles (n = 8 each). Training had no impact on maximal cardiac output, and no differences were observed between the LO group and the HI group (p > 0.05). The a-vO₂diff increased in the HI group only (p < 0.05) and correlated significantly (r = 0.71, p < 0.01) with changes in V̇O_2max across all participants. Muscle capillary density (p < 0.02) and β-hydroxyacyl-CoA dehydrogenase maximal activity (p < 0.05) increased in both groups, with no between-group differences (p > 0.05). Citrate synthase maximal activity (p < 0.01) and type IIA fibre composition (p < 0.05) increased in the LO group only. Collectively, although the heterogeneity in the observed V̇O_2max response following 4 weeks of SIT appears to be attributable to individual differences in systemic vascular and/or muscular adaptations, the markers examined in the current study were unable to explain the divergent V̇O_2max responses in the LO and HI groups.