Pharmacognostic and Pharmacological Evaluation of Hyssopus officinalis L. (Lamiaceae) Collected from Kashmir Himalayas, India

Abstract

Introduction: Hyssopus officinalis L. is a well-known herb for its culinary and medicinal significance. The purpose of this study was to perform the pharmacognostic evaluation. Methods: Physicochemical and phytochemical analysis, HPTLC quantification and in vitro antioxidant and antidiabetic activity were done. Results: Preliminary screening revealed the presence of phytomolecules such as alkaloid (0.99%), tannin (1.75%), sugar (1.96%) and starch (0.68%). Total phenolic and flavonoid content were found to be 2.32% and 1.16% respectively. HPTLC quantification data showed that the content of ferulic acid (0.034%) was higher than caffeic acid (0.0064%) on dry weight basis The IC 50 value for the in vitro DPPH radical scavenging assay was 0.50 g/ml and in vitro anti diabetic assay displayed IC 50 value of 0.8366 mg/ml.