<i>MiR-150-3p</i> targets SP1 and suppresses the growth of glioma cells

Abstract

Glioma has been considered as one of the most prevalent and common malignancy of the nervous system; however, the underlying mechanisms that are responsible for the occurrence and development of glioma still remain largely unknown. Amounting evidence highlights the critical regulatory function of miRNAs in carcinogenesis. Here, we showed that the expression of <i>miR-150-3p</i> was significantly decreased in glioma tissues and cell lines. Suppressed expression of <i>miR-150-3p</i> was associated with the lymph node metastasis of the glioma patients. Overexpression of <i>miR-150-3p</i> significantly inhibited the proliferation of glioma cells. Molecular study uncovered that the transcription factor specificity protein 1 (SP1) was identified as one of the targets of <i>miR-150-3p</i> Highly expressed <i>miR-150-3p</i> in glioma cells significantly decreased both the mRNA and protein levels of SP1. Consistently, the abundance of phosphatase and tension homolog deleted on chromosome ten (PTEN), a negative downstream target of SP1, was increased with the ectopic <i>miR-150-3p</i> Collectively, these results suggested that <i>miR-150-3p</i> suppressed the growth of glioma cells partially via regulating SP1 and possibly PTEN.