Soil N transformation mechanisms can effectively conserve N in soil under saturated conditions compared to unsaturated conditions in subtropical China

Abstract

The connection between moisture and nitrogen (N) transformation in soils is key to understanding N losses, particularly nitrate (NO 3 − ) losses, and also provides a theoretical framework for appropriate water management in agricultural systems. Thus, we designed this study to provide a process-based background for management decision. We collected soil samples from the long-term field experiment in subtropical China, which was designed to examine tobacco and rice rotations under a subtropical monsoon climate. The field experiment was established in 2008 with four treatments: (1) no fertilization as control; (2) N, phosphorus (P), and potassium (K) fertilizers applied at recommended rates; (3) N fertilizers applied at rates 50% higher than the recommended amounts and P and K fertilizers applied at recommended rates; and (4) N, P, and K fertilizers applied at recommended rates with straw incorporated (NPKS). Soil samples were collected during the unsaturated tobacco-cropping season and saturated rice-cropping season and were incubated at 60% water holding capacity and under saturated conditions, respectively. Two 15 N tracing treatments ( 15 NH 4 NO 3 and NH 4 15 NO 3 ) and a numerical modeling method were used to quantify N transformations and gross N dynamics. Autotrophic nitrification was stimulated by N fertilizer both under unsaturated and saturated conditions. The rate of NO 3 − consumption (via immobilization and denitrification) increased under the NPKS treatment under saturated conditions. Secondly, the rates of processes associated with ammonium (NH 4 + ) cycling, including mineralization of organic N, NH 4 + immobilization, and dissimilatory NO 3 − reduction to NH 4 + , were all increased under saturated conditions relative to unsaturated conditions, except for autotrophic nitrification. Consequently, NO 3 − -N and NH 4 + -N concentrations were significantly lower under saturated conditions relative to unsaturated conditions, which resulted in reduced risks of N losses via runoff or leaching. Our results suggest that under saturated conditions, there is a soil N conservation mechanism which alleviates the potential risk of N losses by runoff or leaching.