A new multiplex real‐time TaqMan^®<scp>PCR</scp>for quantification of<i>Mycoplasma hyopneumoniae</i>,<i>M. hyorhinis</i>and<i>M. flocculare</i>: exploratory epidemiological investigations to research mycoplasmal association in enzootic pneumonia‐like lesions in slaughtered pigs

Abstract

This new multiplex qPCR appears to be specific, sufficiently sensitive and repeatable. The validation of this method with field samples guarantees its use for field epidemiological investigations, particularly to gain more insight into the aetiology of the porcine respiratory disease complex.