Development of an in situ‐Product Crystallization (ISPC)‐Concept to Shift the Reaction Equilibria of Selected Amine Transaminase‐Catalyzed Reactions

Abstract

The synthesis of enantiopure amines via amine transaminases involves several challenges including unfavorable reaction equilibria and product inhibition. Described here is a non‐catalytic approach to overcome such problems by using an in situ‐product crystallization (ISPC) to selectively remove a targeted product amine from an amine transaminase‐catalyzed reaction. The continuous removal of the product amine from its reaction solution as a barely soluble salt effectively yields a displacement of the reaction equilibrium towards the products and facilitates a simple downstream processing approach via filtration. The targeted product amine is eventually obtained from the salt, while the counterion compound can be easily recycled.