Nanobody-Directed Specific Degradation of Proteins by the 26S-Proteasome in Plants

Abstract

Here, we present data showing the directed degradation of target proteins recognized by a specific nanobody in transgenic plants. Green fluorescent protein was depleted by a chimeric nanobody fused to a distinct F-box domain, which enables protein degradation <i>via</i> the ubiquitin proteasome pathway. This technique could thus be used to knock out other proteins of interest <i>in planta</i> using specific, high-affinity binding proteins.