Extraction, isolation and identification of flavonoid from Chenopodium album aerial parts

Abstract

<i>Chenopodium album</i> L., (<i>C. album</i>) (family: Chenopodiaceae) is an annual shrub widely grown in Asia, Africa, Europe and North America. It is commonly known as Bathua (in Hindi), pigweed, fat hen or lamb-quarters. The leaves of <i>C. album</i> are applied as a poultice to bug bites, sunstroke, rheumatic joints and as mild laxative. The flavonoids contained in <i>C. album</i> aerial parts were extracted, identified and characterized. Sequential soxhlet extraction was subjected to preliminary phytochemical screening and flavonoid quantification. The results showed that maximum yield of the flavonoid (7.335 mg/g) were obtained from acetone extract. This acetone extract was subjected to flash chromatography for isolation of flavonoid. Characterization of isolated flavonoid was done by UV, IR, 1H & 13C NMR and MS. On the basis of chemical and spectral analysis structure was elucidated as 2-(3, 4-dihydroxyphenyl)-3, 5, 7-trihydroxy-4H-chromen-4-one, a flavonoid.