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Fluorescent probes based on π-conjugation modulation between hemicyanine and coumarin moieties for ratiometric detection of pH changes in live cells with visible and near-infrared channels

EngineeringBiochemistryπ-Conjugation ModulationSingle-molecule DetectionBioimagingRatiometric DetectionChemical ProbePhotophysical PropertyMolecular ImagingBiophysicsNovel Imaging MethodFluorescent Probes