Comparison of fungal fluorescent staining and <scp>ITS rDNA PCR</scp>‐based sequencing with conventional methods for the diagnosis of onychomycosis

Abstract

Fluorescence microscopy has a higher sensitivity for the detection of fungi in nail specimens compared with KOH and can be used as a rapid screening tool. PCR-based sequencing was faster and more sensitive compared with culture and when used in conjunction with fluorescence microscopy resulted in higher efficiency.