<i>C. elegans</i> ADARs antagonize silencing of cellular dsRNAs by the antiviral RNAi pathway

Abstract

Cellular dsRNAs are edited by adenosine deaminases that act on RNA (ADARs). While editing can alter mRNA-coding potential, most editing occurs in noncoding sequences, the function of which is poorly understood. Using dsRNA immunoprecipitation (dsRIP) and RNA sequencing (RNA-seq), we identified 1523 regions of clustered A-to-I editing, termed editing-enriched regions (EERs), in four stages of <i>Caenorhabditis elegans</i> development, often with highest expression in embryos. Analyses of small RNA-seq data revealed 22- to 23-nucleotide (nt) siRNAs, reminiscent of viral siRNAs, that mapped to EERs and were abundant in <i>adr-1;adr-2</i> mutant animals. Consistent with roles for these siRNAs in silencing, EER-associated genes (EAGs) were down-regulated in <i>adr-1;adr-2</i> embryos, and this was dependent on associated EERs and the RNAi factor RDE-4. We observed that ADARs genetically interact with the 26G endogenous siRNA (endo-siRNA) pathway, which likely competes for RNAi components; deletion of factors required for this pathway (<i>rrf-3</i> or <i>ergo-1</i>) in <i>adr-1;adr-2</i> mutant strains caused a synthetic phenotype that was rescued by deleting antiviral RNAi factors. Poly(A)⁺ RNA-seq revealed EAG down-regulation and antiviral gene induction in <i>adr-1;adr-2;rrf-3</i> embryos, and these expression changes were dependent on <i>rde-1</i> and <i>rde-4</i> Our data suggest that ADARs restrict antiviral silencing of cellular dsRNAs.