Concepedia

Publication | Open Access

SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

DOIFull Paper Access

72

Citations

24

References

2018

Year

Yulia G. Ermakova, Valeriy V. Pak, Yulia A. Bogdanova, Alexey A. Kotlobay, Ilia V. Yampolsky, Arina G. Shokhina, Anastasiya S. Panova, R. A. Marygin, Dmitriy B. Staroverov, Dmitry S. Bilan,
Chemical Communications

Abstract

We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pK<sub>a</sub>, which responds to pH changes in different cellular compartments. SypHer3s was successfully utilized for imaging the pH dynamics in mitochondria of living neurons and in quantitative pH measurement in zebrafish embryos.

References

YearCitations

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