Environmental Transformations and Algal Toxicity of Single-Layer Molybdenum Disulfide Regulated by Humic Acid

Abstract

The environmental transformations of nanomaterials are correlated with their behaviors and ecological risks. The applications of single-layer molybdenum disulfide (SLMoS₂) have rapidly developed in environmental fields, but the potential transformations and biological effects of SLMoS₂ remain largely unknown. This study revealed that humic acid (HA, over 10 mg/L) induced the scrolling of SLMoS₂ with light irradiation over a 56-day incubation. The colloidal stability of SLMoS₂ increased, and the aggregation ratio decreased from 0.59 ± 0.07 to 0.08 ± 0.01 nm/min after HA hybridization. Besides, compared with pristine SLMoS₂, the chemical dissolution rate of SLMoS₂ was up to 4.6-fold faster with HA exposure. These results demonstrate that HA affects the environmental fate and transformations of SLMoS₂. SLMoS₂-HA possessed a significantly widened direct band gap (2.06 eV) compared with that of SLMoS₂ (1.8 eV). SLMoS₂ acted as an electronic acceptor from HA, resulting in the separation of electron-hole pairs. Consequently, SLMoS₂-HA exhibited stronger peroxidase-like catalytic activity, which was approximately 2-fold higher than that of SLMoS₂. Moreover, the morphology and layered structure of SLMoS₂ changed, and the damage SLMoS₂ inflicted on microalgae was significantly reduced. This work provides insights into the behaviors and related biological risks of SLMoS₂ in aqueous environments.