Label-free identification carbapenem-resistant <i>Escherichia coli</i> based on surface-enhanced resonance Raman scattering

Abstract

In this study, a surface-enhanced resonance Raman scattering (SERRS) method has been developed for the accurate detection and identification of carbapenem-resistant and carbapenem-sensitive <i>Escherichia coli</i>. A total of 89 human isolates of Enterobacteriaceae, comprising 41 strains of carbapenem-sensitive <i>E. coli</i> (CSEC) and 48 strains of carbapenem-resistant <i>E. coli</i> (CREC), were tested to assess the feasibility of our proposed SERRS method as a clinical tool, and the results showed almost 100% accuracy.