Isolation and characterization of the bioactive metabolites from the soil derived fungus<i>Trichoderma viride</i>

Abstract

The aim of the present study was to evaluate different biological activities of <i>Trichoderma viride</i> fungus (Family Hypocreaceae). <i>Trichoderma viride</i> isolated for the first time from the cucumber soil (rhizosphere). It was tested as antimicrobial, antioxidant and anticancer agent. <i>Trichoderma viride</i> from the cucumber soil (rhizosphere) caused inhibition of the mycelial growth of <i>Fusarium solani, Rhizoctonia solani</i> and <i>Sclerotium rolfsii</i>. Also, the alcoholic extract of the fungal mycelia proved a potent antibacterial activity against <i>Bacillus subtilis, Escherichia</i> coli and <i>Pseudomonas fluorescens</i>. In addition, it exhibited a significant antifungal activity against <i>Candida albicans, Fusarium solani, Fusarium oxysporium, Rhizoctonia solani</i> and <i>Pythium ultimum</i> at 100 µg/disc. Study of the antimicrobial and antioxidant activities of the volatile constituents had been done. The <i>in vitro</i> antioxidant, anticancer and antiviral activities of the isolated proteins, and carbohydrates were determined. Furthermore, the volatile constituents were isolated from fresh mycelia of <i>Trichoderma viride</i> and subjected to GC/MS analysis. Total protein (10%), carbohydrate (19.57%), steroidal (13.95%) and triterpenoidal content (38.34%) were determined in the alcoholic extract of <i>Trichoderma viride</i> mycelia. In conclusion, this fungus showed antioxidant, anticancer, antiviral and antibacterial effects. Further studies must be done to identify the molecules responsible for its effect and to consider its application in the pharmacological and medicinal purposes.