Genetic variants in lncRNA <i>H19</i> are associated with the risk of oral squamous cell carcinoma in a Chinese population

Abstract

To evaluate whether the genetic variants in <i>H19</i> influence the risk of oral squamous cell carcinoma (OSCC) in a Chinese population, a case-control study was conducted to analyze four functional single nucleotide polymorphisms (SNPs) in <i>H19</i>. The cohort comprised of 444 OSCC cases and 984 healthy controls, and the study further evaluated the biological effect by bioinformatics prediction and functional experiments. Two SNPs, rs217727 and rs2839701, were found to be associated with the risk of OSCC [rs217727: odds ratio (OR) = 1.32, 95% confidence interval (CI) = 1.11-1.58, <i>P</i> = 0.002; rs2839701: OR = 1.23, 95% CI = 1.04-1.46, <i>P</i> = 0.019].Bioinformatics predicted that rs2839701 C>G might alter the secondary structure of <i>H19</i>. In addition, rs2839701 C>G inhibited the transcription activity and was correlated with the decreased expression of downstream gene <i>MRPL23-AS1</i> that was downregulated in OSCC. The current results suggested that the SNPs in H19 may play a major role in genetic susceptibility to OSCC.