Construction of Multifunctional Fe₃O₄-MTX@HBc Nanoparticles for MR Imaging and Photothermal Therapy/Chemotherapy

Abstract

To accomplish effective cancer imaging and integrated therapy, the multifunctional nanotheranostic Fe₃O₄-MTX@HBc core-shell nanoparticles (NPs) were designed. A straightforward method was demonstrated for efficient encapsulation of magnetic NPs into the engineered virus-like particles (VLPs) through the affinity of histidine tags for the methotrexate (MTX)-Ni²⁺ chelate. HBc₁₄₄-His VLPs shell could protect Fe₃O₄-MTX NPs from the recognition by the reticuloendothelial system as well as could increase their cellular uptake efficiency. Through our well-designed tactic, the photothermal efficiency of Fe₃O₄ NPs were obviously improved <i>in vitro</i> and <i>in vivo</i> upon near-infrared (NIR) laser irradiation. Moreover, Magnetic resonance imaging (MRI) results showed that the Fe₃O₄-MTX@HBc core-shell NPs were reliable T₂-type MRI contrast agents for tumor imaging. Hence the Fe₃O₄-MTX@HBc core-shell NPs may act as a promising theranostic platform for multimodal cancer treatment.