Antiplasmodial activity of isolated polyphenols from Alectryon serratus leaves against 3D7 Plasmodium falciparum

Abstract

The ethanol extract of <i>Alectryon serratus</i> leaves was successively fractionated in CH₂Cl₂, EtOAc, and n-butanol. EtOAc fraction was fractionated using column chromatography and purified using preparative thin-layer chromatography (TLC). Isolates were studied for their antiplasmodial activity on parasites culture of chloroquine-sensitive 3D7 strain of <i>Plasmodium falciparum</i>. Parasitemia percentages, growth percentages, and inhibition percentages of each group were calculated. The half maximal inhibitory concentration (IC₅₀) values that represent the concentration required to inhibit 50% of <i>Plasmodium</i> growth were calculated from a calibration curve using linear regression. The results suggest that isolates have antiplasmodial activity and are responsible in the antimalarial activity of <i>Alectryon serratus</i> leaves. <b>Abbreviations Used:</b> S.F: Subfraction, EGCG: Epigallocatechingallate, EGC: Epigallocatechin.