Nature Communications · 2017 · 63 citations · 47 references
Life SciencesCell BiologyMolecular BiologyGene ExpressionTranscriptionTranscriptional RegulationNuclear ReceptorsRna Polymerase FunctionRna Polymerase Ii TranscriptionRna Polymerase RecruitmentRna Polymerase RegulationTranscription FactorsDna Binding SitesNuclear Receptor TargetsNuclear Receptor Transcriptional RegulationRna Polymerase Ii ActivationRna Polymerase Ii FunctionRna Polymerase Ii RecruitmentRna Polymerase Ii Regulation
Myosin VI (MVI) has been found to be overexpressed in ovarian, breast and prostate cancers. Moreover, it has been shown to play a role in regulating cell proliferation and migration, and to interact with RNA Polymerase II (RNAPII). Here, we find that backfolding of MVI regulates its ability to bind DNA and that a putative transcription co-activator NDP52 relieves the auto-inhibition of MVI to enable DNA binding. Additionally, we show that the MVI-NDP52 complex binds RNAPII, which is critical for transcription, and that depletion of NDP52 or MVI reduces steady-state mRNA levels. Lastly, we demonstrate that MVI directly interacts with nuclear receptors to drive expression of target genes, thereby suggesting a link to cell proliferation and migration. Overall, we suggest MVI may function as an auxiliary motor to drive transcription.
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Predicting Coiled Coils from Protein Sequences
Andrei N. Lupas, Marc Van Dyke, Jeff Stock · Science · 1991 · 4K citations
Myosin VI is an actin-based motor that moves backwards
Amber L. Wells, Abel W. Lin, Liqiong Chen et al. · Nature · 1999 · 675 citations