Determination of the best conditions for modified biochar immobilized petroleum hydrocarbon degradation microorganism by orthogonal test

Abstract

Modified wheat biochar and modified peanut shell biochar is immobilized carrier, studying its physical and chemical properties, and preparing immobilized microorganisms by adsorption method. The optimum conditions for the preparation of immobilized microorganisms were determined by orthogonal experiment. The effects of biochar types, adsorption time, the amount of biochar and the rotating speed on the amount of bacteria were studied. The results showed the physical and chemical properties of the two modified biochar were beneficial to the adsorption of microorganisms, and when the volume ratio of bacteria liquid and modified peanut shell biochar was 20: 1, stereo adsorption for 2 hours that the optimum immobilization rate was 72.38%.