Radioiodination and biological evaluation of mesalamine as a tracer for ulcerative colitis imaging

Abstract

Abstract This study aims to evaluate newly radioiodinated mesalamine (Mes) as a tracer for ulcerative colitis imaging. Mes was labeled with [ 125 I] with chloramine-T (Ch-T) as the oxidizing agent. Labeling factors such as pH, reaction temperature, reaction time, substrate amount and oxidizing agent amount were investigated to optimize the radiochemical yield (98.5%). The labeled compound was separated and purified using thin layer chromatography (TLC), paper electrophoreses and high performance liquid chromatography (HPLC). The biological distribution indicated that the radioiodinated mesalamine localization was highest (72.28%) in microbial model compared to normal mice (29.25%) at 120 min post injection. So, 125 I-Mes could be considered as a new radiotracer for ulcerative colitis.