Method and key points for isolation of human amniotic epithelial cells with high yield, viability and purity

Abstract

The average yield of obtained hAECs using the new modified method was 190 × 10⁶ cells with a mean viability of 87%, with less than 1% contamination with mesenchymal stem cells (MSCs). The isolated cells were > 95% positive for the epithelial cell markers. The lowest initial plating efficiency of the cells was 80%. Freshly isolated hAECs had the ability to proliferate for 5-6 passages in a standard culture medium.