Calcium-dependent hydrolysis of supported planar lipids was triggered by honey bee venom phospholipase A₂with the right orientation at the interface

Abstract

Hydrolysis of planar phospholipids catalyzed by honey bee venom phospholipase A₂ (bvPLA₂) was studied. Experiments demonstrated that Ca²⁺ ions mediated between the lipids and bvPLA₂, induced reorientation of bvPLA₂, and activated hydrolysis. One of the hydrolysis products, fatty acids, was desorbed, and the other one, lysophospholipids, self-organized at the interface.