The Exometabolome of Two Model Strains of the Roseobacter Group: A Marketplace of Microbial Metabolites

Abstract

Recent studies applying Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) showed that the exometabolome of marine bacteria is composed of a surprisingly high molecular diversity. To shed more light on how this diversity is generated we examined the exometabolome of two model strains of the <i>Roseobacter</i> group, <i>Phaeobacter inhibens</i> and <i>Dinoroseobacter shibae</i>, grown on glutamate, glucose, acetate or succinate by FT-ICR-MS. We detected 2,767 and 3,354 molecular formulas in the exometabolome of each strain and 67 and 84 matched genome-predicted metabolites of <i>P. inhibens</i> and <i>D. shibae</i>, respectively. The annotated compounds include late precursors of biosynthetic pathways of vitamins B₁, B₂, B₅, B₆, B₇, B₁₂, amino acids, quorum sensing-related compounds, indole acetic acid and methyl-(indole-3-yl) acetic acid. Several formulas were also found in phytoplankton blooms. To shed more light on the effects of some of the precursors we supplemented two B₁ prototrophic diatoms with the detected precursor of vitamin B₁ HET (4-methyl-5-(β-hydroxyethyl)thiazole) and HMP (4-amino-5-hydroxymethyl-2-methylpyrimidine) and found that their growth was stimulated. Our findings indicate that both strains and other bacteria excreting a similar wealth of metabolites may function as important helpers to auxotrophic and prototrophic marine microbes by supplying growth factors and biosynthetic precursors.