Studies on the aerobic utilization of synthesis gas (syngas) by wild type and recombinant strains of <i>Ralstonia eutropha</i> H16

Abstract

The biotechnical platform strain Ralstonia eutropha H16 was genetically engineered to express a cox subcluster of the carboxydotrophic Oligotropha carboxidovoransOM5, including (i) the structural genes coxM, -S and -L, coding for an aerobic carbon monoxide dehydrogenase (CODH) and (ii) the genes coxD, -E, -F and -G, essential for the maturation of CODH. The cox_Oc genes expressed under control of the CO₂ -inducible promoter P_L enabled R. eutropha to oxidize CO to CO₂ for the use as carbon source, as demonstrated by ¹³ CO experiments, but the recombinant strains remained dependent on H₂ as external energy supply. Therefore, a synthetic metabolism, which could be described as 'carboxyhydrogenotrophic', was established in R. eutropha. With this extension of the bacterium's substrate range, growth in CO-, H₂ - and CO₂ -containing artificial synthesis gas atmosphere was enhanced, and poly(3-hydroxybutyrate) synthesis was increased by more than 20%.