Computational fluid dynamics simulation of an industrial P. chrysogenum fermentation with a coupled 9-pool metabolic model: Towards rational scale-down and design optimization

Abstract

Metabolic-hydrodynamic simulation to predict yield loss due to substrate gradient. Novel design approach for representative scale-down simulators. Use of simulations to assess effect of reactor design on process yield. Experimental verification of penicillin production rate in fed-batch simulation. Prediction of emerging population heterogeneity in fed-batch simulation.