Xanthomonas oryzae pv. oryzae Inoculation and Growth Rate on Rice by Leaf Clipping Method

Abstract

Bacterial blight caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae (Xoo</i>) is one of the most serious bacterial diseases and a major impediment to the increase of rice yield. Appropriate methods for inoculation of <i>Xoo</i> and disease scoring are necessary to investigate the nature of the disease and the mechanism of plant resistance to the pathogen. As the most-widely grown crop in the worldwide, rice yield plays an important role in food security. Uncovering mechanisms of plant-pathogen interaction of rice and <i>Xoo</i> will help develop rice plants that are more resistant to disease caused by <i>Xoo</i>. Here we describe our validated and efficient methods for inoculation of <i>Xoo</i> and disease scoring.